Citation from DataCite
ORCID:0000-0002-2776-1403
Affiliation: Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg (Germany), GRID: 433014.1
Vaida, Shrijana
Affiliation: Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg (Germany), GRID: 433014.1
Paraje Sanchez, Eva
Affiliation: University of Lleida (Crop and Forest Sciences Department, Associated Unit EEAD-CSIC), GRID: 15043.33
Buchen, Caroline
Affiliation: Johann Heinrich von Thünen Institute (Germany), GRID: 11081.39
Huth, Vytas
Affiliation: University of Rostock (Faculty of Agricultural and Environmental Sciences), GRID: 10493.3f
Augustin, Jürgen
Affiliation: Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg (Germany), GRID: 433014.1
GEMET:
Other: Gross primary productivity; ecosystem respiration; autotrophic respiration; heterotrophic respiration; net ecosystem carbon balance; light inhibition of leaf respiration
- To date, it is still unclear whether leaf level autotrophic respiration (LRa), inhibited during light, affects autotrophic respiration (Ra), and estimates of an ecosystems net CO2 exchange (NEE)
- Using manual closed chambers, we investigated within two alfalfa pot experiments: (1) whether Ra is inhibited during daytime at leaf (LRa) and ecosystem level (Ra) ; as well as (2) how this influences consistency and accuracy of NEE estimates, derived using the three most widely applied gas exchange measurement methods
- The first experiment confirmed a light inhibition of daytime LRa but not Ra of 12% to 32%. The second experiment however, revealed a clear influence of light inhibited daytime LRa on Ra and NEE estimates by cross-checking cumulative Reco, GPP (gross primary productivity) and NEE estimates with independently measured net primary productivity (NPP).
- Two opposing systematic biases were identified, causing this: (1) an overestimation of NEE due to an overestimation of daytime and/or nighttime Reco; (2) an underestimation of NEE due to an unbalanced impact on the dependency functions used to derive Reco and GPP, which are required to estimate NEE
- Hence, if not corrected for, commonly derived NEE of terrestrial ecosystems will be systematically biased
(Paper submitted to New Phytologist)
IsDocumentedBy DOI: https://doi.org/10.4228/ZALF.2011.339
References DOI: 10.1016/j.agrformet.2014.09.005
Table Measured_daytime_Ra_d_and nighttime_Ra_n_leaf respiration (first pot experiment):
Table Weather_data (second pot experiment):
Table Measures_CO2_fluxes_1st_pot_exp (first pot experiment) and
Table Measured_CO2_fluxes_2nd_pot_exp (second pot Experiment)
These tables are nearly the same except for the last two columns.
Table Modelled_CO2_exchange (second pot experiment):
Reference for the model: 10.4228/ZALF.2011.339
Table Simulated_CO2_exchange:
View Sample Data
Table Measured_daytime_Ra_d_and nighttime_Ra_n_leaf respiration
Table Weather_data
Table Measured_CO2_fluxes_1st_pot_exp
Table Measured_CO2_fluxes_2nd_pot_exp
Table Modelled_CO2_exchange
Table Simulated_CO2_exchange
Download Link
Download dataset
Note: On Nov 25, 2020 we updated Title and Description, fluxes in table Measured_daytime_Ra_d_and nighttime_Ra_n_leaf respiration and added the table Measured_CO2_fluxes_1st_pot_exp (now including correction for changes in Ci following Ayub et al. 2011).
This data by ZALF is licensed under the Creative Commons Attribution 4.0
| Column name | Unit | Description | Instrument | Method | Reference |
| Date | yyyy-mm-dd | date of measurement | - | - | - |
| Time | hh:mm:ss | time of measurement | - | - | - |
| Cuvette_area_coverage | % | Cuvette covered by leaf | - | measured with scale paper and fine scales followed by measurements in relation to cuvette surface | |
| Air_temperature_inside | °C | Average air temperature measured inside the cuvette of the GFS-3000 | sensor of GFS-3000 | - | - |
| Air_humidity_inside | % | Average air humidity in % measured inside the cuvette of the GFS-3000 | sensor of GFS-3000 | - | |
| Ra_d_LAISK | µmol m-2 s-1 | CO2-flux in µmol m-2 s-1 derived from gas exchange measurments using the GFS-3000 and Laisk method, respectively | Portable Gas-Exchange and Fluorescence System (GFS-3000, Heinz Walz GmbH) | Leaf-level gas exchange measurement and analysis | Laisk AK (1977) Kinetics of photosynthesis and photorespiration in C3 plants. Nauka Moscow (in Russian) |
| Ra_d_KOK | µmol m-2 s-1 | CO2-flux in µmol m-2 s-1 derived from gas exchange measurements using the GFS-3000 and Kok method, respectively | Portable Gas-Exchange and Fluorescence System (GFS-3000, Heinz Walz GmbH) | Kok B (1948) A critical consideration of the quantum yield of Chlorella-photosynthesis. Enzymologia 13, 1-56 | |
| Ra_n | µmol m-2 s-1 | CO2-flux in µmol m-2 s-1 derived from gas exchange measurements using the GFS-3000 and Kok method, respectively | Portable Gas-Exchange and Fluorescence System (GFS-3000, Heinz Walz GmbH) | Leaf-level gas exchange measurement and analysis | Kok B (1948) A critical consideration of the quantum yield of Chlorella-photosynthesis. Enzymologia 13, 1-56 |
| Ra_d_reduction_LAISK | % | Light inhibition of Ra(d): Reduction (in %) in daytime (autotrophic) leaf-level respiration due to light inhibition derived from gas exchange measurements using the GFS-3000 and Laisk method, respectively | - | calculation of measured values | - |
| Ra_d_reduction_KOK | % | Light inhibition of Ra(d): Reduction (in %) in daytime (autotrophic) leaf-level respiration due to light inhibition derived from gas exchange measurements using the GFS-3000 and Kok method, respectively | - | calculation of measured values | - |
Table Weather_data (second pot experiment):
| Column name | Unit | Description | Instrument | Method | Reference |
| Date_Time | yyyy-mm-dd | date and time of measurement | - | - | - |
| Air_temp | °C | Air temperature in °C measured in half hour steps | Combisensor KS 550 connected to weather station WS550 | - | - |
| PAR | µmol m-2 s-1 | Photosynthetic active radiation (400 nm - 700 nm; outside chamber) measured in half hour steps | Combisensor KS 550 connected to weather station WS550 | - | - |
Table Measures_CO2_fluxes_1st_pot_exp (first pot experiment) and
Table Measured_CO2_fluxes_2nd_pot_exp (second pot Experiment)
These tables are nearly the same except for the last two columns.
| Column name | Unit | Description | Instrument | Method | Reference |
| Date_Time | yyyy-mm-dd | date and time of measurement | - | - | - |
| Pot | - | Measured pot (e.g., "pot_1” to “pot_2", etc.) | - | - | - |
| ID | - | Measurement ID | - | - | - |
| Chamber_type | - | Chamber type (0=opaque;1=transparent) | - | - | - |
| Measured_Reco_NEE_flux | µmol m-2 s-1 | Measured Reco or NEE flux (according to "Chamber_type") | gas analyzer (LI-840, LI-COR Biosciences) | calculation from manual chamber measurements | https://doi.org/10.1016/j.agrformet.2014.09.005 |
| FLUX_start_concentration | ppmv | CO2 starting concentration | gas analyzer (LI-840, LI-COR Biosciences) | calculation from manual chamber measurements | https://doi.org/10.1016/j.agrformet.2014.09.005 |
| Slope_linear_regression | - | Slope of linear regression applied on measured CO2 concentration change over measurement time of a measurement | - | Calculated according to Hoffmann et al. 2015 as refered in 10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| P_value_slope | - | p-value of the slope of the applied linear regression | - | Calculated according to Hoffmann et al. 2015 as refered in 10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| R_square_flux | - | r2 of the applied linear regression | - | Calculated according to Hoffmann et al. 2015 as refered in 10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Mean_PAR | µmol m-2 s-1 | Mean photosynthetic active radiation of the identified flux | - | Calculated according to Hoffmann et al. 2015 as refered in 10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Temperature_IN | °C | Mean air temperature (inside chamber) for the identified flux | - | Calculated according to Hoffmann et al. 2015 as refered in 10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Temperature_variation | °C | Air temperature variation for the identified flux | - | Calculated according to Hoffmann et al. 2015 as refered in 10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Temperature_OUT | °C | Mean air temperature (outside chamber) for the identified flux | - | Calculated according to Hoffmann et al. 2015 as refered in 10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
Table Modelled_CO2_exchange (second pot experiment):
Reference for the model: 10.4228/ZALF.2011.339
| Column name | Unit | Description | Instrument | Method | Reference |
| Date_Time | yyyy-mm-dd | date and time of measurement | - | - | - |
| Pot | - | Measured pot (e.g., "pot_1” to “pot_2", etc.) | - | - | - |
| Reco | g C m-2 0.5h-1 | Modelled Recoflux | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| GPP | g C m-2 0.5h-1 | Modelled GPP | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| NEE | g C m-2 0.5h-1 | Modelled NEE flux | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Best_fit_int_rev_up | - | Best-fit temperature for Reco modelling ("IN", "OUT" or "10cm") of the next campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Best_fit_int_rev_down | - | Best-fit temperature for Reco modelling ("IN", "OUT"or "10cm") of the previous campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Rref_OUT...IN...10cm...int_rev_up | - | Calculated Rref-parameter of best-fit temperature fit for Reco during next campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Rref_OUT...IN... 10cm...int_rev_down | - | Calculated Rref-parameter of best-fit temperature fit for Reco during previous campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| EO_OUT...IN... 10cm_int_rev_up | - | Calculated E0-parameter of best-fit temperature fit for Reco during next campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| EO_OUT...IN...10cm_int_rev_down | - | Calculated E0-parameter of best-fit temperature fit for Reco during previous campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Flux_AVG_int_rev_up | µmol m-2 s-1 | Calculated Mean Reco flux of the next campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Flux_AVG_int_rev_down | µmol m-2 s-1 | Calculated Mean Reco flux of the previous campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| a_GPP_int_rev_up | - | Calculated Alpha parameter of GPP fit with photosynthetic active radiation (PAR) during next campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| a_GPP_int_rev_down | - | Calculated Alpha parameter of GPP fit with photosynthetic active radiation (PAR) during previous campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Gpmax_int_rev_up | - | Calculated GPmax parameter of GPP fit with photosynthetic active radiation (PAR) during next campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
| Gpmax_int_rev_down | - | Calculated GPmax parameter of GPP fit with photosynthetic active radiation (PAR) during previous campaign | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45
10.1016/j.agrformet.2014.09.005; 10.4228/ZALF.2011.339 |
Table Simulated_CO2_exchange:
| Column name | Unit | Description | Instrument | Method | Reference |
| Pot | - | Measured pot (e.g., "Pot_1” to “Pot_2", etc.) | - | - | - |
| rad_change | % | Intended Ra(d) change in percent (%) | - | - | - |
| NPP | g C pot-1 | Measured NPP in g C pot-1 | Elemental Analyzer (EA) Flash 2000 HT (Thermo Fisher Scientific, Germany) | measured | - |
| Reco | g C pot-1 | Simulated cumulative Reco flux in C pot-1 | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 Using a reduced Reco based on changed Ra(d) |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45; 10.4228/ZALF.2011.339 |
| GPP | g C pot-1 | Simulated cumulative GPP flux in C pot-1 | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 Using a reduced Reco based on changed Ra(d) |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45; 10.4228/ZALF.2011.339 |
| NEE_Rh | g C pot-1 | Simulated cumulative NEE(-Rh) flux in C pot-1 | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 Using a reduced Reco based on changed Ra(d) |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45; 10.4228/ZALF.2011.339 |
| NEE_Rh_NPP | g C pot-1 | C-balance in g C pot-1 | - |
Calculated according to Hoffmann et al. 2015 as refered
in
10.4228/ZALF.2011.339 Using a reduced Reco based on changed Ra(d) |
Hoffmann, M. et al.(2015) Automated modeling of ecosystem
CO2 fluxes based on periodic closed chamber measurements:
a standardized conceptual and practical approach. Agric.
For. Meteorol. 200, 30–45; 10.4228/ZALF.2011.339 |
Table Weather_data
Table Measured_CO2_fluxes_1st_pot_exp
Table Measured_CO2_fluxes_2nd_pot_exp
Table Modelled_CO2_exchange
Table Simulated_CO2_exchange
This data by ZALF is licensed under the Creative Commons Attribution 4.0