Citation from DataCite
ORCID: 0000-0002-0966-8600
Affiliation: Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg (Germany), GRID: 433014.1
Müller, Marina
Affiliation: Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg (Germany), GRID: 433014.1
Researcher: Koch, Mathias
Researcher: Lentzsch, Peter
HostingInstitution: Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg (Germany), GRID: 433014.1
Mycotoxigenic fungal pathogens Fusarium and Alternaria are a leading cause for loss in cereal production. During their growth in wheat-ears, they oppose bacterial antagonists such as Pseudomonas. Studies on these groups’ interactions often neglect the infection process’s temporal aspect and the associated priority effects. Here, we focus on how the first colonizer affects the subsequent ones. In a climatic chamber experiment, wheat-ears were sprayed successively with a pair of the strains A. tenuissima At625, F. graminearum Fg23, or Pseudomonas simiae Ps9. Over three weeks, microbial abundances and mycotoxin concentrations were analyzed and visualized via Self Organizing Map with Sammon Mapping (SOM-SM). All three strains revealed different characteristics and strategies to deal with co-inoculation: Fusarium, as the first colonizer, suppressed the establishment of Alternaria and Pseudomonas. Nevertheless, primary inoculation of Alternaria reduced all of the Fusarium toxins and stopped Pseudomonas from establishing. Pseudomonas showed priority effects in delaying and blocking the production of the fungal mycotoxins. The SOM-SM analysis visualized the competitive strengths: Fg23 ranked first, At625 second, Ps9 third. Our findings of species-specific priority effects in a natural environment and the role of the mycotoxins involved are relevant for developing biocontrol applications.
Methods:
In a climate chamber experiment wheat plants were primarily inoculated with Fusarium/Alternaria or Pseudomonas, after one week an additional isolate different from the first one was applied. In the following third week, the inoculation of the second week was repeated. Before each new inoculation, a part of the previous variety was harvested. All harvested wheat-ears were dried and ground before further analysis (-> qPCR and HPLC).
FunderIdentifier (GRID): 424150.6
AwardNumber: DFG GRK 2118/1
AwardTitle: BioMove Research Training Group
AwardURI: https://www.biomove.org/
| Column name | Unit | Description |
| Sample_ID | Sample_ID: X: first inoculant (1 week after first inoculation) XX:first inoculant + second inoculant (2 week after first inoculation) XXX: first inoculant + second inoculant + third inoculant (3 week after first inoculation) with A (=Alternaria alternata), F (=Fusarium graminearum), P (=Pseudomonas simae) and C (=Control) for X | |
| F_val1 | gene copies /g extracted DNA | Abundances of the genus Fusarium_ Measurement 1 |
| F_val2 | gene copies /g extracted DNA | Abundances of the genus Fusarium_ Measurement 2 |
| P_val1 | gene copies /g extracted DNA | Abundances of Pseudomonas_ Measurement 1 |
| P_val2 | gene copies /g extracted DNA | Abundances of Pseudomonas_ Measurement 2 |
| A_val1 | gene copies /g extracted DNA | Abundances of the genus Alternaria_ Measurement 1 |
| A_val2 | gene copies /g extracted DNA | Abundances of the genus Alternaria_ Measurement 2 |
| DON | mg/kg | deoxynivalenol measurement |
| 3-Ac-DON | mg/kg | 3-acetyl deoxynivalenol measurement |
| 15-Ac-DON | mg/kg | 15-acetyl deoxynivalenol measurement |
| DON-3G | mg/kg | deoxynivalenol-3-glucoside measurement |
| NIV | mg/kg | nivalenol measurement |
| ZEN | mg/kg | zearalenone measurement |
| ALT | mg/kg | altenuene measurement |
| AOH | mg/kg | alternariol measurement |
| AME | mg/kg | alternariol monomethyl ether measurement |
| TEA | mg/kg | tenuazonic acid measurement |
